estrogenic activity of some phytoestrogens on bovine oxytocin and thymidine kinase-ere promoter through estrogen receptor-α in mda-mb 231 cells
نویسندگان
چکیده
background: phytoestrogens, a group of plant-derived polyphenolic compounds have recently come into considerable attention due to the increasing information on their potential adverse effects in human health. some of phytoestrogens show estrogenic activity that may be carcinogenic for human. in the present study, we investigated the transcriptional effects of variety of phytoestrogens on the bovine oxytocin and the thymidine kinase-ere promoter by estrogen receptor α in mda-mb 231 breast cancer cell line. materials and methods: cells were seeded for transfections into 12- well plates at a density of 100000 cells per well were transfected with a total of 3 μg of plasmid dna using calcium phosphate coprecipitation. estrogen and some phytoestrogens (naringenin, 8-prenyl-naringenin and 6-( 1, 1 - dimethylallyl ) naringenin were used for the stimulation of transfected cells. results: findings of our study clearly demonstrated the subtype-selective activation of estrogen receptor (er)α and (er)β by the p hytoestrogen naringenin (activating estrogen receptor β) and its substituted forms 8-prenyl-naringenin and 6-( 1, 1 - dimethylallyl ) naringenin (activating estrogen receptor α) , on the ere-controlled promoter as well as on the oxytocin gene promoter. conclusion: the study revealed that some p hytoestrogen s show estrogenic activity by classical or non-classical mechanisms as well as exhibit estrogenic activity by undetermined mechanisms in transfected mda-mb 231 cell line.
منابع مشابه
Estrogenic Activity of Some Phytoestrogens on Bovine Oxytocin and Thymidine Kinase-ERE Promoter through Estrogen Receptor-α in MDA-MB 231 Cells
Background: Phytoestrogens, a group of plant-derived polyphenolic compounds have recently come into considerable attention due to the increasing information on their potential adverse effects in human health. Some of phytoestrogens show estrogenic activity that may be carcinogenic for human. In the present study, we investigated the transcriptional effects of variety of phytoestrogens ...
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BACKGROUND: Some of metal ions as environmental pollutants show estrogenic activity. This xenostrogenic compounds can be caused carcinogenicity in organs. The mechanism of carcinogenicity of metal ions is not clarified. OBJECTIVES: In this study, we investigated the Transcriptional effects of variety of metal ions on the bovine oxytocin and the thymidine kinase-ERE promoter by estrogen receptor...
متن کاملtranscriptional effects of metal ions on the bovine oxytocin and the thymidine kinase-ere promoter through the estrogen receptor a in mda-mb 231 breast cancer cell line
background: some of metal ions as environmental pollutants show estrogenic activity. this xenostrogenic compounds can be caused carcinogenicity in organs. the mechanism of carcinogenicity of metal ions is not clarified. objectives: in this study, we investigated the transcriptional effects of variety of metal ions on the bovine oxytocin and the thymidine kinase-ere promoter by estrogen receptor...
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عنوان ژورنال:
research in molecular medicineجلد ۲، شماره ۳، صفحات ۲۹-۳۶
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